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Transfection of bovine cell culture with bovine herpesvirus 4 DNA obtained by cell nuclear extraction


This review summarizes most recent information on the bovine cytomegalovirus BHV-4. We have compared the subcellular localizations of gE and gG and examined the cell-to-cell adherence of bovine kidney (MDBK) cells infected with BHV-1 mutants lacking gE or gG. Bovine mastitis caused due to virus is often neglected as bacterial infections are held mainly responsible for the disease. In both PCR assays, all 31 BHV4 strains examined were scored positive, whereas 14 unrelated viruses scored negative. Like other herpesviruses, BoHV-4 establishes persistent infections in its natural host [5, 6] and in an experimental host, the rabbit [7]. (iii) Enzymatic digestion of cell surface heparan sulfate but not of chondroitin sulfates A, B, and C reduced the binding of the virus to the cells, and rendered the cells partially resistant to infection. The existence of three groups of strains, which were designated as genotypes 1, 2 and 3, is described.

The study confirmed the presence of BoHV-1 in cattle farms with reproductive problems in Sudan. The possibility of obtaining infectious viral DNA using this method may facilitate the construction of recombinant viruses. Specifically, through the use of cotransfection experiments with deleted or mutated viral DNA sequences, the infectious clones isolated could provide the basis for an increased understanding of BHV-4 viral gene expression, replication and pathogenesis.