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Experimental transplacental infection of pregnant dogs with canine herpesvirus. – PubMed


This paper presents the entire nucleotide sequence of the thymidine Kinase (TK) gene of canine herpesvirus (CHV). When nucleotide sequence of the mutated gD of YP11mu strain (gD(YP11mu)) was compared with that of gDs of other CHV strains, gD(YP11mu) lacked 12 nucleotides encoding 4 amino acids, NKTI, including one predicted potential N-linked glycosylation site and no other change was found in other regions. The G protein expressed by the recombinant CHV was processed and transported to the cell surface as in RV infected cells, and showed the same biological activities such as low pH dependent cell fusion and hemadsorption. Results—Dogs in the infection group developed bilateral, mild to moderate conjunctivitis that reached maximal intensity on PIDs 7 to 10. Thirteen live-born and 2 stillborn pups derived from 3 bitches by cesarean section died within 9 days of birth. Get a printable copy (PDF file) of the complete article (844K), or click on a page image below to browse page by page. Fourteen of the fetal placentas had variable degree of necrotizing lesions.

Seroprevalence was independent of age, gender or colony size. Lesions were present in the walls of allantoic blood vessels. Intranuclear inclusions were evident in degenerating trophoblast cells and in the cells of maternal and fetal blood vessels. Infective virus and viral antigen were detected in the various organs of pups and in the trophoblastic cells of junctional zone. Virus also was found in the walls of maternal and fetal blood vessels of the placental tissues. The results strongly indicated that transplacental transmission of CHV may occur in pregnant dogs at a late stage of gestation.